Gastric cancer

Gastric cancer (GC) is one of the common types of cancer in Vietnam. Over 50% of GCs are diagnosed at an unresectable stage. Deficiency in mismatch repair proteins (MMR) leading to microsatellite instability (MSI-H) is a crucial prognostic factor currently under investigation in these patients.

6p viharuno 03-01-2025 0 0   Download

Single-nucleotide polymorphisms (SNP) are common genetic changes in the human genome. In recent time, many scientists have shown their interests in studies on SNPs’ roles in the pathological risk especially in cancer and metabolic diseases.

27p tamynhan0 15-06-2020 18 1   Download

Porcine heart cytoplasmic malate dehydrogenase (s-MDH) is a dimeric protein (2 · 35 kDa). We have studied equilibrium unfolding and refolding of s-MDH using activity assay, fluorescence, far-UV and near-UV circular dichroism (CD) spectroscopy, hydrophobic probe-1-anilino8-napthalene sulfonic acid binding, dynamic light scattering, and chromatographic (HPLC) techniques. The unfolding and refolding transitions are reversible and show the presence of two equilibrium intermediate states.

11p system191 01-06-2013 39 4   Download

Visual arrestin is converted from a ÔbasalÕ state to an ÔactivatedÕ state by interaction with the phosphorylated C-terminus of photoactivated rhodopsin (R*), but the conformational changes in arrestin that lead to activation are unknown. Small-angle X-ray scattering (SAXS) was used to investigate the solution structure of arrestin and characterize changes attendant upon activation. Wild-type arrestin forms dimers with a dissociation constant of 60 lM.

9p system191 01-06-2013 53 3   Download

The plastid transcription kinase (PTK), a component of the major RNA polymerase complex from mustard chloroplasts, has been implicated in redox-mediated regulation of plastid gene expression. A cloning strategy to define the PTK gene(s) resulted in the isolation of a full-length cDNA for a protein with overall high homology with the a subunit of cytosolic casein kinase (CK2) that contained an N-terminal extension for a putative plastid transit peptide.

9p system191 01-06-2013 46 4   Download

PII-like proteins are signal transduction proteins found in bacteria, archaea and eukaryotes. They mediate a variety of cellular responses. A second PII-like protein, called GlnK, has been found in several organisms. In the diazotroph Herbaspirillum seropedicae, PII protein is involved in sensing nitrogen levels and controlling nitrogen fixation genes. In this work, the crystal structure of the unliganded H. seropedicae PII was solved by X-ray diffraction. H. seropedicae PII has a Gly residue, Gly108 preceding Pro109 and the main-chain forms a b turn. ...

8p system191 01-06-2013 37 5   Download

We have examined the effects of widely used stress-inducing agents on protein synthesis and on regulatory components of the translational machinery. The three stresses chosen, arsenite, hydrogen peroxide and sorbitol, exert their effects in quite different ways. Nonetheless, all three rapidly ( 30 min) caused a profound inhibition of protein synthesis. In each case this was accompanied by dephosphorylation of the eukaryotic initiation factor (eIF) 4E-binding protein 1 (4E-BP1) and increased binding of this repressor protein to eIF4E. ...

10p system191 01-06-2013 44 4   Download

Business Unit Cell Cybernetics, Plant Research International, Wageningen, the Netherlands; 2University of Wu¨rzburg, Chair of Food Chemistry, Germany; 3Laboratory of Plant Physiology, Wageningen University, the Netherlands Citrus limon possesses a high content and large variety of monoterpenoids, especially in the glands of the fruit flavedo. The genes responsible for the production of these monoterpenes have never been isolated.

12p system191 01-06-2013 42 7   Download

A clear difference in the enthalpy changes derived from spectroscopic and calorimetric measurements has recently been shown. The exact interpretation of this deviation varied from study to study, but it was generally attributed to the non-two-state transition and heat capacity change. Although the temperature-dependent thermodynamics of the duplex formation was often implied, systemic and extensive studies have been lacking in universally assigning the appropriate thermodynamic parameter sets.

10p system191 01-06-2013 37 4   Download

Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, Belgium; 2Institut de Pharmacologie et Biologie Structurale, UMR-CNRS 5089, Toulouse Cedex, France; 3Rega Institute, Laboratory of Molecular Immunology, Katholieke Universiteit Leuven, Belgium Fruits of elderberry (Sambucus nigra) express small quantities of a type-2 ribosome-inactivating protein with an exclusive specificity towards the NeuAc(a2,6)Gal/GalNAc disaccharide and a unique molecular structure typified by the occurrence of a disulfide bridge between the B-chains of two adjacent protomers....

10p system191 01-06-2013 43 3   Download

Microcalorimetry and UV-vis spectroscopy were used to conduct thermodynamic and kinetic investigations of the scission of calf thymus DNA catalyzed by bleomycin A5 (BLM-A5) in the presence of ferrous ion and oxygen. The molar reaction enthalpy for the cleavage, the Michaelis– Menten constant for calf thymus DNA and the turnover number of BLM-A5 were calculated by a novel thermokinetic method for an enzyme-catalyzed reaction to be )577 ± 19 kJÆmol)1, 20.4 ± 3.8 lM and 2.28 ± 0.49 · 10)2 s)1, respectively, at 37.0 °C. This DNA cleavage was a largely exothermic reaction....

9p system191 01-06-2013 31 4   Download

gp130 is the common signal transducing receptor subunit of interleukin (IL)-6-type cytokines. gp130 either homodimerizes in response to IL-6 and IL-11 or forms heterodimers with the leukemia inhibitory factor (LIF) receptor (LIFR) in response to LIF, oncostatin M (OSM), ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1) or cardiotrophinlike cytokine resulting in the onset of cytoplasmic tyrosine phosphorylation cascades. The extracellular parts of both gp130 and LIFR consist of several Ig-like and fibronectin type III-like domains.

11p system191 01-06-2013 45 4   Download

School of Biosciences, University of Birmingham, Edgbaston, Birmingham, UK; 2Shifa College of Medicine, Islamabad, Pakistan.Curcumin is a compound derived from the spice, tumeric. It is a potent inhibitor of the SERCA Ca2þ pumps (all isoforms), inhibiting Ca2þ-dependent ATPase activity with IC50 values of between 7 and 15 mM . It also inhibits ATPdependent Ca2þ-uptake in a variety of microsomal membranes, although for cerebellar and platelet microsomes, a stimulation in Ca2þ uptake is observed at low curcumin concentrations (,10 mM )....

10p system191 01-06-2013 37 5   Download

This study reports the identification and characterization of the regulatory subunit, TbRSU, of protein kinase A of the parasitic protozoon Trypanosoma brucei. TbRSU is coded for by a single copy gene. The protein contains an unusually long N-terminal domain, the pseudosubstrate site involved in binding and inactivation of the catalytic subunit, and two C-terminally located, closely spaced cyclic nucleotide binding domains.

10p system191 01-06-2013 37 4   Download

Departamento de Fisiologıa de la Nutricion, Instituto Nacional de Ciencias Medicas y Nutricion ‘Salvador Zubiran’, Mexico; ´ ´ ´ ´ ´ Departamento de Patologıa Experimental, Instituto Nacional de Ciencias Medicas y Nutricion ‘Salvador Zubiran’,Mexico; 3Department of Biochemistry, Wake Forest University Medical Center, Winston-Salem, North Carolina, USA Branched chain amino-acid aminotransferase (BCAT) activity is present in fetal liver but the developmental pattern of mitochondrial BCAT (BCATm) expression in rat liver has not been studied.

8p system191 01-06-2013 48 6   Download

Frog p26olf is a novel S100-like Ca21-binding protein found in olfactory cilia. It consists of two S100-like domains aligned sequentially, and has a total of four Ca21-binding sites (known as EF-hands). In this study, to elucidate the mechanism of Ca21-binding to each EF-hand (named EF-A, -B, -C and -D from the N-terminus of p26olf), we examined Ca21-binding in wild-type p26olf and also in its mutants in which a glutamate at the –z coordinate position within each Ca21-binding loop was substituted for a glutamine.

8p system191 01-06-2013 29 4   Download

BTG/Tob family proteins, which are characterized by similarities in their N-terminal BTG/Tob homology domains, control cell growth negatively. Among the BTG/ Tob family members, BTG2/TIS21/PC3 proteins have been reported to have short lives and to be degraded by the proteasome. However, the mechanisms regulating the stabilities of other BTG/Tob family proteins have not yet been clarified.

9p research12 01-06-2013 31 4   Download

Four substrate analogs, 4-(2-naphthyloxy)-2-butyn-1-amine (1), 1,4-diamino-2-chloro-2-butene (2), 1,6-diamino-2,4hexadiyne (3), and 2-chloro-5-phthalimidopentylamine (4) have been tested as inhibitors against mammalian, plant, bacterial, and fungal copper-containing amine oxidases: bovine plasma amine oxidase (BPAO), equine plasma amine oxidase (EPAO), pea seedling amine oxidase (PSAO), Arthrobacter globiformis amine oxidase (AGAO), Escherichia coli amine oxidase (ECAO), and Pichia pastoris lysyl oxidase (PPLO).

14p research12 01-06-2013 43 6   Download

The human multidrug resistance-associated protein (MRP1) is an ATP-dependent efflux pump that transports anionic conjugates, and hydrophobic compounds in a glutathione dependent manner. Similar to the other, well-characterized multidrug transporter P-gp, MRP1 comprises two nucleotide-binding domains (NBDs) in addition to transmembrane domains. However, whereas the NBDs of P-gp have been shown to be functionally equivalent, those of MRP1 differ significantly.

9p research12 01-06-2013 29 4   Download

This study investigated the enzymatic function of two putative plant GPXs, GPXle1 from Lycopersicon esculentum and GPXha2 from Helianthus annuus, which show sequence identities with the mammalian phospholipid hydroperoxide glutathione peroxidase (PHGPX). Both purified recombinant proteins expressed in Escherichia coli show PHGPX activity by reducing alkyl, fatty acid and phospholipid hydroperoxides but not hydrogen peroxide in the presence of glutathione.

7p research12 01-06-2013 37 4   Download