Báo cáo khoa học: Functional analysis of cell-free-produced human endothelin B receptor reveals transmembrane segment 1 as an essential area for ET-1 binding and homodimer formation
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The functional and structural characterization of G-protein-coupled recep-tors (GPCRs) still suffers from tremendous difficulties during sample preparation. Cell-free expression has recently emerged as a promising alter-native approach for the synthesis of polytopic integral membrane proteins and, in particular, for the production of G-protein-coupled receptors.
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