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Báo cáo khoa học: Functional dissection of the Schizosaccharomyces pombe Holliday junction resolvase Ydc2: in vivo role in mitochondrial DNA maintenance
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The crystal structure of theSchizosaccharomyces pombe Holliday junction resolvase Ydc2 revealed significant struc-tural homology with the Escherichia coliresolvase RuvC but Ydc2 contains a small triple helical bundle that has no equivalent in RuvC.Two of thea-helices that form this bundle show homology to a putative DNA-binding motif known as SAP.To investigate the biochemical function of the triple-helix domain, truncated Ydc2 mutants were expressed inE.coliand in fission yeast.
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