Báo cáo khoa học: In vitro selection and characterization of a stable subdomain of phosphoribosylanthranilate isomerase
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The (ba)8-barrel is the most common enzyme fold and it is capable of cata-lyzing an enormous diversity of reactions. It follows that this scaffold should be an ideal starting point for engineering novel enzymes by directed evolution. However, experiments to date have utilized in vivo screens or selections and the compatibility of (ba)8-barrels with in vitro selection methods remains largely untested. We have investigated plasmid display as a suitablein vitro format by engineering a variant of phosphoribosylanth-ranilate isomerase (PRAI) that carried the FLAG epitope in active-site-forming loop 6. ...
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