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Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity
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Novel chromogenic substrates for endo-b-galactosidase were designed on the basis of the structural features of keratan sulfate. Galb1-4GlcNAcb1-3Galb1-4GlcNAcb-pNP (2),which consists of two repeating units ofN-acetyl-lactosamine,was synthesized enzymatically by consecutive additions of GlcNAc and Gal residues top-nitrophenyl b-N-acetyllactosaminide. In a similar manner,Glc-NAcb1-3Galb1-4GlcNAcb-pNP (1),GlcNAcb1-3Galb1-4Glcb-pNP (3),Galb1-4GlcNAcb1-3Galb1-4Glcb-pNP (4), Galb1-3GlcNAcb1-3Galb1-4Glcb-pNP (5),and Galb1-6GlcNAcb1-3Galb1-4Glcb-pNP (6) were synthesized as analogues of2. Endo-b-galactosidases released GlcNAcb-pNP or Glcb-pNP in an endo-manner from each substrate....
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