intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:11

29
lượt xem
2
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

Novel chromogenic substrates for endo-b-galactosidase were designed on the basis of the structural features of keratan sulfate. Galb1-4GlcNAcb1-3Galb1-4GlcNAcb-pNP (2),which consists of two repeating units ofN-acetyl-lactosamine,was synthesized enzymatically by consecutive additions of GlcNAc and Gal residues top-nitrophenyl b-N-acetyllactosaminide. In a similar manner,Glc-NAcb1-3Galb1-4GlcNAcb-pNP (1),GlcNAcb1-3Galb1-4Glcb-pNP (3),Galb1-4GlcNAcb1-3Galb1-4Glcb-pNP (4), Galb1-3GlcNAcb1-3Galb1-4Glcb-pNP (5),and Galb1-6GlcNAcb1-3Galb1-4Glcb-pNP (6) were synthesized as analogues of2. Endo-b-galactosidases released GlcNAcb-pNP or Glcb-pNP in an endo-manner from each substrate....

Chủ đề:
Lưu

Nội dung Text: Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
2=>2