Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies
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Using 0.4Mimidazole citrate buffer (pH 7.5) containing 0.1 mML-cysteine, homodimeric starch phosphorylase from Corynebacterium calluane(CcStP) was dissociated into native-like folded subunits concomitant with release of pyridoxal 5¢-phosphate and loss of activity. The inactivation rate of CcStP under resolution conditions at 30Cwas, respectively, four- and threefold reduced in two mutants, Arg234fiAla and Arg242fiAla, previously shown to cause thermostabilization of CcStP [Griessler, R., Schwarz, A., Mucha, J.&Nidetzky, B. ...
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