Báo cáo khoa học: Surface exposed amino acid differences between mesophilic and thermophilic phosphoribosyl diphosphate synthase
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The amino acid sequence of 5-phospho-a-D-ribosyl 1-diphosphate synthase from the thermophile Bacillus caldolyticusis 81% identical to the amino acid sequence of 5-phospho-a-D-ribosyl 1-diphosphate synthase from the mesophileBacillus subtilis.Nevertheless the enzyme fromthe two organisms possesses very different thermal properties. TheB. caldolyticusenzyme has optimal activity at 60–65C and a half-life of 26 min at 65C, compared to values of 46Cand60sat65C, respectively, for theB. subtilis enzyme. ...
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