Báo cáo lâm nghiệp: " Growth, mineral nutrient content and nitrogen metabo- ism in Laccaria laccata-inoculated and non-inoculated Douglas fir seedlings during their growth period"
Chia sẻ: Nguyễn Minh Thắng
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Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: Growth, mineral nutrient content and nitrogen metabo-
ism in Laccaria laccata-inoculated and non-inoculated Douglas fir seedlings during their growth period...
Nội dung Text: Báo cáo lâm nghiệp: " Growth, mineral nutrient content and nitrogen metabo- ism in Laccaria laccata-inoculated and non-inoculated Douglas fir seedlings during their growth period"
nitrogen metabo-
Growth, mineral nutrient content and
lism in Laccaria laccata-inoculated and non-inoculated
Douglas fir seedlings during their growth period
M. Chalot, B. Botton J. Banvoy
Université de Nancy I, Facult6 des Sciences, Laboratoire de Physiologie Végétale et Forestibre,
BP 239, 54506 Vandœuvre-Iès-Nancy, France
needles and roots were removed immediately
Introduction
after collection for amino acid, protein and
enzyme determinations. Aspartate aminotrans-
ferase (AAT) activity was determined spectro-
Enhanced caused by ecto-
plant growth photometrically and proteins were measured
inoculation in the nursery is
mycorrhizal using a colorimetric method. Amino acids were
analyzed using an HPLC procedure. Total N
well documented: thus Laccaria strains
was measured by a microKjeldahl method, total
have proven to be very efficient for im-
P by a colorimetric method and K and Mg by
proving growth of various conifers (Le atomic absorption spectrophotometry.
Tacon and Bouchard, 1986). The investi-
gations described herein were carried out
to obtain information on physiological and
biochemical modifications that can explain
Results
such stimulation. The present study com-
pared the seasonal changes in protein
and amino acid levels, aspartate amino-
the end of the growing period, L. lac-
By
transferase activity and nutrient content in
cata-inoculated seedlings were significant-
both L. laccata-inoculated and non-inocu-
ly higher (not shown) and had accumulat-
lated Douglas fir grown in a forest nursery.
ed more than 2-fold the fresh matter of the
controls (Table I). In expanding shoots, N,
P, K and Mg concentrations decreased
rapidly until mid-August (Fig. 1 A, B, C, D)
and tended to increase after growth
Materials and Methods
ceased. No significant difference was
found in nutrient analysis of stems and
After 1 yr of growth in the nursery, 12 seedlings roots between treated and untreated
from both untreated and treated plots were col-
plants (not shown). By contrast, statistical-
lected at random, bimonthly from May to Sep-
ly significant (ANOVA, P 0.05) changes
=
tember. Root and top fresh weights and heights
were observed for leaf nutrient analysis
were measured for each seedling. Parts of the
between treatments and, more particularly, Concerning protein amounts and AAT
by the end of the growing season (Fig. 1A, activity levels, high and significant differ-
ences (ANOVA, P 0.05) were found in
B, C, D). =
foliar analysis between inoculated seed- As a hypothesis, we might propose that
lings and controls: leaves of inoculated lower AAT activity combined with a higher
Douglas fir accumulated higher protein proline amount in non-inoculated Douglas
amounts (Fig. 1 F) and showed greater fir characterized seedlings in stress-induc-
AAT capacities (Fig. 1 E). By contrast, ing situations. AAT is often considered as
there was no difference in root analysis an indicator of physiological activity in
between treatments (Fig. 1 E, F). plants affected by various internal or ex-
ternal factors (Cooper and Hill-Cofting-
Alanine, aminobutyric acid, glutamine,
ham, 1974) and proline accumulation de-
aspartic acid, arginine, proline and glu-
scribed as a response to environmental
tamic acid were the major components of
stress factors (Bode ef al., 1985). Inocula-
the free amino acid pools in root and leaf
tion with efficient mycorrhizal strains might
tissues. Alanine and glutamine in both root
provide a way to suppress or at least to
and leaf tissues appeared to be in higher
attenuate stress-inducing situations.
amounts in L. laccata-inoculated Douglas
fir, whereas proline preferentially accumu-
lated in non-inoculated seedlings (Table 11).
References
Discussion and Conclusion
Bode J., Kuhn H.P. & Wild A. (1985) The accu-
mulation of proline in needles of damaged
spruce. Forstwiss. Centralbl. 104, 353-360
Growth measurements reaffirmed the well-
D.R. &. Hill-Cottingham D.G. (1974)
Cooper
established importance of L. laccata strain
Glutamic dehydrogenase and glutamic oxaloa-
238 for improving seedling growth of
cetate transaminase in apple tree. Physiol.
Douglas fir (Le Tacon and Bouchard, Plant. 31, 193-199
1986). Leaf nutrient content following ino- Harley J.L. (1969) In: The Biology of Mycorrhi-
culation also confirmed the importance of Leonard Hill, London
zae.
ectomycorrhizae for nutrient uptake, as Krishna K.R. & E3agyara D.J. (1983) Changes in
reviewed by Harley (1969). the free amino nitrogen and protein fractions of
groundnut caused by inoculation with VA
Our results also suggested that inocula-
mycorrhizas. Ann. Bot 51, 399-401
tions caused changes in the biochemical
Le Tacon F. & Bouchard D. (1986) Effects of dif-
processes of the host tissues as demon-
ferent ectomycorrhizal fungi on growth of larch,
strated earlier (Nemec and Meredith,
Douglas fir, Scots pine and Norway spruce
1981; Krishna and Bagyara, 1983). Thus, seedlings in fumigated nursery soil. Acta
alanine, proline and glutamine contents, Oecol. Appl. 7, 389-402
protein amounts and AAT activity levels Nemec S. & Meredith F. (1981) Amino acid
differed between controls and treated content of leave:> in mycorrhizal and non mycor-
plants. rhizal citrus root stocks. Ann. Bot. 47, 203-222
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