zunia.vn

Tuyển sinh 2024 dành cho Gen-Z

zunia.vn

» Luận Văn - Báo Cáo

» Báo cáo khoa học

Báo cáo lâm nghiệp: "In vitro propagation of Prosopis P. cineraria and P. juliflora) species"

Chia sẻ: Nguyễn Minh Thắng | Ngày: | Loại File: PDF | Số trang:3

Báo xấu

41
lượt xem 3
download

Download Vui lòng tải xuống để xem tài liệu đầy đủ

Tuyển tập các báo cáo nghiên cứu về lâm nghiệp được đăng trên tạp chí lâm nghiệp Original article đề tài: In vitro propagation of Prosopis P. cineraria and P. juliflora) species...

Chủ đề:

Bình luận(0) Đăng nhập để gửi bình luận!

Lưu

Nội dung Text: Báo cáo lâm nghiệp: "In vitro propagation of Prosopis P. cineraria and P. juliflora) species"

In vitro propagation of Prosopis species (P. chilensis, P. cineraria and P. juliflora) C.A. Batchelor D. Yao, M.J. Koehler P.J.C. Harris Department of Biological Sciences, Coventry Polytechnic, Priory Street, Coventry CV1 5FB, U.K. immersed in an anti-oxidant solution (100 mg/I Introduction citric acid, 50 mg/I ascorbic acid, 100 mg/I poly- vinyl pyrrolidone) for 15 min. Murashige and Skoog medium was used with 8 g/I agar, 30 g/I The genus Prosopis has attracted con- sucrose, 1.6 g/l glutamine and 81 combinations siderable interest for forestry in arid areas. of plant hormones, including kinetin (K) although normally propagated by seed, (0.05-15 mgA), benzylamino purine (BA) vegetative propagation of selected Proso- (0.05-15 mg/I), indole acetic acid (IAA) (1-10 mg/I), indole butyric acid (IBA) pis plants from variable populations and (1-15 mg/I) and naphthalene acetic acid (NAA) from natural hybrids may also be desir- (1-15 mg/I). Between 4 and 16 explants were able. Propagation by cuttings has been placed on each medium and incubated at 25°C reported (e.g., Felker and Clark, 1981). ). with a 16 h pho’toperiod and a photon flux den- The use of tissue culture techniques to sity of 65-200 !E/m2 for 51 d. regenerate plants from nodal explants has also been reported for P. cineraria (Goyal and Arya, 1984), P. tamarugo and P. Results chilensis (Jordan and Balbao, 1985), and P, alba (Tabone et al., 1986). In this study, tissue culture media were evaluated for Without hormones, results for rooting per- root initiation, shoot proliferation and shoot centage, mean number of shoots/explant growth of P. chilensis, P. cineraria and P. node and mean number of nodes/regen- juliflora. erated shoot were, P. chilensis, 0%, 0.8, 1.2, P. cineraria, 13%, 0.8, 1.0, and P. juli- flora, 6%, 0.4, 1.0. A summary of the most successful hormone treatments is given in Materials and Methods Table I. High levels of BA induced shoot proliferation of P. chilensis with 15 mg/l Explants with 1 or 2 nodes were taken from the BA, 5 mg/l NAA giving the highest mean 6 nodes of the main stem and youngest of 4.3 shoots/node. Similar levels of K branches of 3-12 mo old, greenhouse-grown were much less effective for shoot prolif- stock plants. Explants were surface sterilized in eration. Shoot growth of P. chilensis was 70% industrial methylated spirit for 1 min and greatest (5 nodes/shoot) with 0.05 mg/I K, 5% (v/v) sodium hypochlorite for 5-10 min, and
IBA. 0.05 mg/I BA with 3 mg/I IBA, the greatest (75%) percentage of rooted 3 mg/I mg/I K, with 3 or 15 mg/I IBA 1 0.05 explants. or or induced rooting. 0.05 mg/I K, 3 mg/I IBA Shoot proliferation of P. cineraria was the number (2.5) of also promoted by high levels of BA, greatest gave and 1 mg/I IBA 10 mg/I BA, 5 mg/I NAA resulting in 3 mg/I K, 15 roots/explant
shoots/node. K failed to induce multiple trations of BA, but not of K in combination shoot production in P. cineraria. Shoot with auxins, promote shoot proliferation probably by stimulating axillary bud growth was greatest (3 nodes/shoot) with 3 mg/I K, 23 mg/l NAA but 1 mg/I BA, growth. Medium to high concentrations of 3 mg/I IBA gave reduced leaf abscission. auxin in combination with low to medium Rooting of P. cineraria was obtained only cytokinin concentrations promote shoot with combinations of K and IBA (0.05 mg/I growth. The results for R chilensis and P. cineraria provide the basis for a possible K, 1-15 mg/I IBA, and 1 mg/I K, 15 mg/I IBA), the greatest number of roots (2.8/ micropropagation system consisting of explant) being initiated with 1 mg/l K, shoot proliferation, shoot growth and root- 15 mg/I IBA and the highest rooting per- ing stages, an!! this system is being eva- luated. Further work with P. juliflora is centage (75%) being with 0.05 mg/I K, required to optimize culture conditions for 15 mg/I IBA. each stage. Results for P. juliflora were less conclu- sive. In very few cases were multiple shoots obtained and no treatment gave a shoots/node. Shoot growth mean of >1.5 Acknowledgmients of P. juliflora was greatest (3 nodes/shoot) with 0.05 mg/I K, 15 mg/I IBA. 10 mg/I K, This research was funded by the Henry Double- 1 mg/I IAA gave the greatest leaf reten- day Research Association. tion. Root initiation by P. juliflora was most successful with K in combination with IBA. 0.05 mg/I K, 15 mg/I IBA was the best treatment (75% rooted explants, 5.6 References roots/explant). Rooted plantlets of each species were transferred to compost in a Clark P.R. (1981) Felker P. & of Rooting mes- greenhouse with 100% survival after 3 quite (Prosopis) cuttings. J. Range Manage. 34, months. 466-468 Goyal Y. & Arya H.C. (1984) Tissue culture of desert trees: 1. Clonal multiplication of Proso- pis cineraria by bud culture. J. Plant Physiol. 115, 183-189 Discussion and Conclusion Jordan M. & Balbao O. (1985) In vitro regenera- tion of Prosopis tamarugo Phil. and Prosopis chilensis (Mol.) Stuntz from nodal sections. The relative ease of micropropagation in Gartenbauwisse.nchaft 50, 138-142 this study was P. chilensis > P. cineraria > Tabone T.J., Felker P., Bingham R.L., Reyes I. P. juliflora. The results for all 3 species & Loughrey S. (1986) Techniques in the shoot show a similar pattern. In general, IBA multiplication of the leguminous tree Prosopis promotes rooting and K is less inhibitory to alba clone B For. Ecol. Manage. 16, 191- . 50 V 2 root production than is BA. High concen- 200

CÓ THỂ BẠN MUỐN DOWNLOAD

LV.01: Bộ Luận Văn Thạc Sĩ Quản Trị Kinh Doanh MBA

165 tài liệu

2069 lượt tải

THÔNG TIN

TRỢ GIÚP

HỖ TRỢ KHÁCH HÀNG

Theo dõi chúng tôi

Chịu trách nhiệm nội dung:

Nguyễn Công Hà - Giám đốc Công ty TNHH TÀI LIỆU TRỰC TUYẾN VI NA

LIÊN HỆ

Địa chỉ: P402, 54A Nơ Trang Long, Phường 14, Q.Bình Thạnh, TP.HCM

Hotline: 093 303 0098

Email: support@tailieu.vn

Giấy phép Mạng Xã Hội số: 670/GP-BTTTT cấp ngày 30/11/2015 Copyright © 2022-2032 TaiLieu.VN. All rights reserved.