Bioprospecting of antibiofilm potential of Piper Nigrum, Piper Betle and Coscinium Fenestratum on Escherichia coli

Genetic language for social behavior in bacteria is mediated through Quorum sensing. This collective group behavior posses obvious advantages for the bacteria to adapt to suitable growth modes by migrating to amicable environmental conditions which makes them highly virulent and detrimental to the environment. 80% of the uncomplicated UTIs that occur in healthy individuals are due to uropathogenic E. coli which are the leading cause of morbidity. Current study investigates antibiofilm activity of methanol extracts from Coscinium fenestratum, Piper betle and Piper nigrum against clinically isolated, MDR E. coli. LC / MSMS screened the herbal bioactive compounds. The extracts exhibited a MIC in a range of 0.1mg/mL- 0.15mg/mL. Reduction of EPS and rhamnolipids was found to be 79%, 68% and 62.0% respectively by C. fenestratum, P. betle and P. nigrum. Berberine and Eugenol can be used as quorum inhibitors for attenuation of MDR E. coli at sub inhibitory concentration of 50µg/mL.