Characterization of enteroviruses in irrigating water in Egypt and in clinical samples with the assessment of ethanolic extract of nigella sativa in response to these viruses

This study aimed to determine enteroviruses using different PCR methods and also studying the effect of ethanolic extract of Nigella Sativa on isolated viruses in vitro. we performed reverse transcription-PCR (RT-PCR), nested PCR and cell culture-PCR followed by real time PCR to monitor the occurrence of enteric viruses in in 23 samples collected from irrigation water sites in El-Giza and El-Menofya Governorates from March 2017 to May 2017; fourteen samples from five rural sites in El-Menofya which no sanitation systems service these regions and nine samples from three urban sites in El-Giza served by sanitation systems were investigated.