Comparative assessment and optimisation of different DNA extraction methods in lesser yam (Dioscorea esculenta)

The effectiveness of genotyping for any genetic studies relies on the quantity and quality of DNA isolated. The DNA isolation procedures differs for different crop species depending upon the phytochemical composition of the tissue used for isolation of DNA. The polyphenol abundance in Lesser yam interferes in the isolation of high quality DNA. The quantitative and qualitative assessment of DNA isolated using different extraction methods therefore becomes a priority. The selection of accurate isolation method becomes absolutely essential to obtain PCR amplification. In this study, five DNA extraction methods were compared in terms of quantity, quality/purity, time consumed, integrity and functionality.