Comparison of the efficacy of serum gonadotrophin, estrodiol-17β, estrous buffalo serum and buffalo follicular fluid supplementation on invitro cytoplasmic maturation rate of buffalo oocytes

An experiment was conducted to compare the efficacy of serum gonadotropin @50 and 100IU/ml, estrodiol-17β @0.5 and 1μg/ml, EBS @5 and 10% and BFF @5 and 10% supplementation on in vitro cytoplasmic maturation rate of buffalo oocytes. Ovaries were collected from buffaloes slaughtered at local abattoir. Oocytes were aspirated and good and excellent quality oocytes were matured in basic maturation media TCM199 supplementing above mentioned supplements separately.