De-rhamnosylation of hesperidin to hesperitin-7-o-glucoside by alkali tolerant α-L-rhamnosidase from Fusarium poae MTCC-2086

An alkali tolerant α-L-rhamnosidase from the culture filtrate of a fungal strain, Fusarium poae MTCC-2086 has been purified to homogeneity. The procedure involved concentration by ultrafiltration and cation-exchange chromatography on carboxymethyl cellulose. The purified enzyme gave a single protein band corresponding to molecular mass of 51.0 kDa in SDS-PAGE analysis showing that the enzyme preparation was pure. The native PAGE analysis of the purified enzyme also gave single protein band confirming the purity of the enzyme preparation.