Design of high-oleic tobacco (Nicotiana tabacum L.) seed oil by CRISPR-Cas9- mediated knockout of NtFAD2–2

Tobacco seed oil could be used as an appropriate feedstock for biodiesel production. However, the high linoleic acid content of tobacco seed oil makes it susceptible to oxidation. Altering the fatty acid profile by increasing the content of oleic acid could improve the properties of biodiesel produced from tobacco seed oil.