Detection of staphylococcus aureus and methicillin resistant staphylococcus aureus (MRSA) from bovine raw milk by PCR

Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) has been considered to be a major cause of healthcare infections worldwide and poses a major threat to public health. It is also one of the main etiological agents which is responsible for clinical and subclinical mastitis in dairy herds. This study was designed to investigate the occurrence of S. aureus and Methicillin resistant Staphylococcus aureus (MRSA) from bovine raw milk by Polymerase chain reaction (PCR) targeting thermonuclease (nuc) and mecA gene.