Development of a method for genotyping the MTHFR rs1801133 polymorphism in Vietnamese, using the polymerase chain reaction with confronting two-pair primers

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To develop a method using polymerase chain reaction with confronting two-pair primers (PCR-CTPP) to genotype MTHFR rs1801133 polymorphism in Vietnamese people. Methods: Fifteen DNA samples from a group of Vietnamese people were used to evaluate this assay. The Sanger sequencing method was used to identify the standard genotypes of the rs1801133 polymorphism.

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