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Effect of ionic liquid on the enzymatic synthesis of cello-oligosaccharides and their assembly into cellulose materials
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Imidazolium-based ionic liquids are important solvents for the processing of natural cellulose. Little is known about their use in synthesizing cellulose via bottom-up polymerization of β-1,4-D-glucosyl chains in solution. Here, we analyzed cellodextrin phosphorylase-catalyzed synthesis of cello-oligosaccharides, and the subsequent spontaneous self-assembly of the chains, in the presence of cellulose-dissolving ionic liquid, 1,3-dimethylimidazolium dimethyl phosphate ([Dmim]DMP) or 1-ethyl-3-methylimidazolium acetate ([Emim]OAc).
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