Evaluation of single step TaqMan real-time PCR assay lateral to conventional RT-PCR and antigen-capture ELISA for pre-clinical detection of classical swine fever virus

In the present study, a fluorogenic-probe hydrolysis (TaqMan)-reverse transcriptase real time PCR assay (RT-qPCR)was evaluated parallel to conventional RT-PCR and antigen capture ELISA to detect Classical swine fever virus (CSFV) in the pre-clinical phase of the disease.