First DNA barcodes and records of Sciaridae (Insecta, Diptera) from Romania

Sciaridae or the dark-winged fungus gnats fauna of southern and eastern Europe is very poorly known. A total of 29 species were identified from Romania, of which 20 species are reported here for the first time from the country. An updated checklist of darkwinged fungus gnats (Sciaridae) of Romania is provided. DNA barcodes of 39 specimens belonging to 21 species were successfully sequenced, of which some were previously unknown or undetected.