Fixation of lethal dose50 and effect of mutagens in M1 generation under laboratory condition

The current study was conducted to find out the LD50 dose, shoot length and root length induced by both gamma rays and EMS in blackgram variety VBN 4, under laboratory condition. The investigation was carried out by exposing the seeds with different doses of gamma rays (10, 20, 30, 40, 50, 60, 70, 80 and 90kR) and various concentrations of EMS (30, 40, 50, 60 and 70mM) along with the control.