Impact of culture media and duration on antioxidant and alpha-glucosidase inhibitory activities of methanol extract from Graphis handelii mycobiont

This study investigated the influence of culture media and culture time on the antioxidant and α-glucosidase inhibitory activities of the methanol extract obtained from the mycobiont of Graphis handelii. Different culture media, including MIX, PDA, and SAB4%, were employed to culture the mycobiont, and the biomass was harvested during different culture periods (4, 8, and 12 weeks).