In vitro organogenesis from nodal derived callus in carnation (Dianthus caryophyllus L.) cv. ‘Master’ and assessment of genetic fidelity of acclimatized plantlets

The in vitro raised plantlets were successfully acclimatized under ex vitro conditions and grew well in the glasshouse. All the regenerated plantlets appeared normal with respect to morphological characteristics with 100% survival rate. Somaclonal variation in tissue culture is a common phenomenon which makes it mandatory to check for genetic stability of plants. The genetic variation in the in vitro raised plants was confirmed using Inter Simple Sequence Repeats(ISSR) markers. Comparison of the bands with the mother plant revealed that the genetic variation frequency reached 6.54 % using fifteen ISSR markers.