Isolation of lipase-producing yeasts from industrial oily residues in different culture media

The main goal of this work was to collect industrial oily residues and isolate lipolytic yeasts by selecting the best strain in relation to lipolytic and esterification activities and investigate the increase of production in shake flasks. A total of 78 yeasts were isolated on Yeast Malt agar and 44 strains with lipase-producing potential pre-selected on tributyrin agar. The yeast identified as Yarrowia lipolytica showed the highest lipolytic and esterification activities, reaching 2.67 U/mL and 464.65 U/g, respectively, at 48 h. Medium 4 showed major lipolytic (2.23 U/mL) and esterification (324.98 U/g) activity, concomitantly.