Karyomorphology, ploidy analysis, and flow cytometric genome size estimation of Medicago monantha populations

Karayomorphological analysis and monoploid genome size (2Cx DNA) of 12 Iranian Medicago monantha populations are reported. Flow cytometric analysis were conducted on freshly collected seeds, using Solanum lycopersicum cv. Stupicke (2C DNA = 1.96 pg), as the internal reference standard. All populations were diploid with variable degrees of mixoploidy and two different chromosome numbers within each population.