Molecular breeding and variability analysis of high beta carotene Introgressed BC1F2 line of maize

Among all the micro nutrient deficiencies, World Health Organization (WHO) had identified Vitamin A deficiency as the more dangerous and widespread micronutrient deficiencies in the world. In order to address this global problem, biofortification of Maize is considered to be a cost effective and sustainable approach. The present study was carried out to raise and identify high β- carotene maize lines of BC1F2 (UMI 1200 × HP 467-15)- C15-S79 population by phenotyping using HPLC along with genotyping of crtRB1 allele. The randomly selected 10 seeds of selected BC1F2 cob were analyzed for β-carotene content through HPLC and the results showed confirmation of improvement in β-carotene content of 3.00 µg/g respectively.