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Mutation allele frequency threshold does not affect prognostic analysis using nextgeneration sequencing in oral squamous cell carcinoma
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With the development of sequencing technologies, there may be some disputes on sequencing analysis. The aim of this study was to investigate different allele frequency thresholds of mutations in targeted genes on prognostic analyses using a panel of cancer associated gene exons (CAGE) in oral squamous cell carcinoma (OSCC).
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