Optimization of multiplex RT-PCR for M1, M23, and M23X splice variants of AQP4 and β-actin transcripts in Dalton’s lymphoma mouse tissues

Multiplex reverse transcriptase–polymerase chain reaction (MRT-PCR) is a method of choice for efficient and simultaneous analysis of gene expression at the transcript level with limited amounts of tissues; however, it is less common than other methods due to certain limitations.