Optimization of production, purification and lyophilisation of cellobiose dehydrogenase by Sclerotium rolfsii

The enzyme cellobiose dehydrogenase (CDH) can be used to oxidize lactose to lactobionic acid. As Sclerotium rolfsii is known to be a good producer of CDH, the aim of this paper was to simplify its production and secondly to systematically study its purification aiming for a high yield.