Production of bacterial cellulose films by gluconoacetobacter xylinus for lipase immobilization

Results showed that increasing in rotational speed from 0 rpm to 200 rpm converted cellulose-producing cells to non-cellulose-producing ones, leading to a significant decline in BC film formation. The increase in initial inoculum size from 0.01 g/L to 0.1 g/L reduced sugar concentration and surface area of the medium, and therefore inhibiting the formation of film-like cellulosic biomass.