Ranjini Tantry Nagesh, et al

A flow cytometric protocol amenable to the crop was standardized by comparing four buffer compositions, three sample sizes and four incubation periods for isolated nuclei using Pisium sativum cv. Citrad (2C=9.09pg) as an internal reference standard. The study revealed significant differences for 2C DNA content among the accessions ranging from 5.73pg to 6.25pg, with 8.79% difference. The mean 2C DNA content of tall accessions has6.07pg/2C and 5.89pg/2C in dwarfs, corresponding to 1C genome size of 2968.23 Mb and 2880.21Mb, respectively.