Rapid electrochemical detection of single and double nucleotide mismatches (mutations) of sequences from HIV samples

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Rapid electrochemical detection of single and double nucleotide mismatches (mutations) of sequences from HIV samples

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For the first time, the detection of DNA sequences, related to HIV (found in AIDS patients), using Methylene Blue (MB) as the redox indicator was performed. The voltametric signals have been investigated on bared chitosan (CS) modified; probe immobilized (DNA single strand) and target-hybridized (DNA double strand) electrodes sequentially by means of Cyclic Voltammetry (CV) and Square Wave Voltammetry (SWV). The response of hybridization between the probe and perfectly complementary, double-base mismatch, single-base mismatch of analyzed targets were clearly identified. The response of CS modified electrode with non-complementary target sequence and the response of CS-free electrode (adsorption only) with complementary target were used as control references to confirm whether the detected signals correspond trully to the hybridization and respond selectively to the targets.

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Nội dung Text: Rapid electrochemical detection of single and double nucleotide mismatches (mutations) of sequences from HIV samples

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