Towards the development of protein expression by inducible ecdysone system

The ecdysone inducible system was generated for checking the toxicity of target gene which was transected into the cells. The transcription unit, modified ecdysone receptor (IND) was cloned into vector pcDNA-Dest40 to form the new inducible expression plasmid pDest40-IND via cloning technique. A clear band with size about 487bp of PCR product indicated the success of amplification IND promoter.