16s rrna gene PCR assay

Between 2018 and 2020, 989 clinical specimens from pigs showing clinical signs of a variety of swine diseases in 27 provinces in China were sampled and submitted for further testing. Nested PCR targeting the 16S rRNA gene of Mycoplasma hyopneumoniae and subsequent sequencing were used to analyse these specimens. Mycoplasma hyopneumoniae-positive samples were assayed by multilocus sequence typing (MLST).

6p vidarwin 22-02-2022 18 1   Download

This shows an intraspecies difference between Bacteroides species in the Philippines and Japan. This difference is a result of variations in the gut microbiome such as host species, diet, and geography. Results of this study will expand the present sequence database of Bacteroides from ducks that can be used in identifying sources of fecal pollution in the environment.

7p mudbound 10-12-2021 9 0   Download

A zoonotic disease Leptospirosis is caused by pathogen of the genus Leptospira and it is an emerging global public health problem. In the present study a total of 266 cattle blood and sera samples were collected from the towns and villages of Nagpur, Wardha, Bhandara Gadchiroli and Durg districts during December 2017 to 2018 and tested for the presence of Leptospira. These samples were from randomly selected herds with history of repeated breeding, abortion, reproductive disorders, etc. also including some apparently healthy animals.

7p nguathienthan2 19-12-2019 17 0   Download

Brucellosis is one of the world’s major zoonoses that affect wide range of domesticated as well as wild animals. Despite the eradication program of brucellosis, the disease is still endemic among cattle, buffaloes, sheep and goats. In many developing countries including India, Brucella melitensis is of much concern due to heavy economic losses to animal husbandry sector as well as humans. The present study was carried out to detect and characterize B.

7p nguaconbaynhay1 04-12-2019 16 0   Download