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Acetohydroxyacid synthase
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Somatic cell selection in plants allows the recovery of spontaneous mutants from cell cultures. When coupled with the regeneration of plants it allows an effective approach for the recovery of novel traits in plants.
10p
vihamax2711
21-04-2020
11
0
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In this study, the recombinant catalytic subunit of AHAS from H. influenza (Hin-AHAS) was expressed in Escherichia coli. The purified Hin-AHAS protein exhibited a molecular weight of approximately 63 kDa on SDS-PAGE gel. The apparent Vmax and Km values of the purified Hin-AHAS were determined to be 0.236 U/mg protein and 2.503 mM pyruvate, respectively.
7p
jangni
16-04-2018
31
3
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Acetohydroxyacid synthase (AHAS, EC 4.1.3.18) catalyses the first step in branched-chain amino acid biosynthesis and is the target for sulfonylurea and imidazolinone herbicides, which act as potent and specific inhibitors. Mutants of the enzyme have been identified that are resistant to particular herbicides.However, the selectivityof thesemutants towards various sulfonylureas and imidazolinones has not been determined systematically.
10p
fptmusic
16-04-2013
24
2
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Acetohydroxyacid synthase (AHAS; EC 2.2.1.6) is the first enzyme in the biosynthetic pathway of the branched-chain amino acids. It catalyzes the conversion of two molecules of pyruvate into 2-acetolactate or one mole-cule of pyruvate and one molecule of 2-ketobutyrate into 2-aceto-2-hydrox-ybutyrate.
9p
vinaphone15
27-02-2013
48
2
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