Acetylene reduction assay

In this study, 8 diazotrophic strains were isolated from rhizosphere soils and root nodules of legume and non-legume plants in Vietnam. Five of the eight isolates showed noticeable nitrogen fixation capability as determined via acetylene reduction assay, from 3.11 to 17.9 nmol/mL/h.

11p vimulcahy 18-09-2023 5 3   Download

Rice cultivation is taken in flooded soil condition. This creates an anaerobic environment and harbours different group of anaerobes. The continued submergence of soil provides a reducing environment and effects a build-up of anaerobic ecosystem in which two unique processes namely methane emission and reductive transformation of nitrogen occur. But the role played by specific group of anaerobes with respect to nitrogen transformation is still obscure. A study was undertaken to assess the different groups of anaerobes in nitrogen transformation in flooded rice soils.

10p trinhthamhodang1215 23-09-2020 11 2   Download

Nitrogen (N) is an essential element for the growth and yield of wheat. Some endophytic bacteria can fix N2 from the air and convert to nitrogen compounds that can be utilized by plants. In this study, endophytic bacteria were isolated from one-month-old seedlings of 13 wheat genotypes growing without nitrogen fertilizer in the ICAR –IARI field. A total of 23 diazotrophic endophytes were isolated from 13 wheat genotypes used. Nitrogenase enzyme activities of endophytes were measured by acetylene reduction assay (ARA).

8p angicungduoc6 20-07-2020 19 2   Download