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Agrobacterium infiltration
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Forest trees have important economic and ecological value. As a model tree, poplar has played a significant role in elucidating the molecular mechanisms underlying tree biology. However, a lack of mutant libraries and time-consuming stable genetic transformation processes severely limit progress into the functional characterization of poplar genes.
19p
vijichea2711
28-05-2021
7
1
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A simple method for transient expression of reporter gene in Brinjal leaves through agroinfiltration
Brinjal (Solanum melongena L.) is one of the most important vegetable crops in India. In spite of the docility of brinjal towards genetic transformation through Agrobacterium, development of stable transgenic lines in brinjal is a cost, labour as well as time consuming process. On the other hand, the agro infiltration process has been optimized in different crops for the rapid analysis of transgene through transient expression. The process has also gained importance in synthetic biological applications for rapid production of therapeutic biomolecules in plants.
7p
chauchaungayxua6
26-06-2020
24
1
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Phaseolus vulgaris is one of the most extensively studied model legumes in the world. The P. vulgaris genome sequence is available; therefore, the need for an efficient and rapid transformation system is more imperative than ever.
14p
viuchiha2711
21-04-2020
14
1
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Bài viết tiến hành chuyển đoạn gen mã hóa cho protein interleukin-7 (IL-7) đã được nhân dòng vào vector pRTRA để tạo cassette 35S-IL7-cMyc-histag-100xELP; sau đó ghép nối vào vector chuyển gen pCB301 và biến nạp vào vi khuẩn Agrobacterium tumefaciens.
7p
vitheseus2711
28-10-2019
16
1
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