Analytical biochemistry

The work presented in this thesis illustrates strategies to improve the detection of glucose by NanoZymes. This was done by first determining the NanoZyme activity of Cu and bimetallic nanoparticles of Cu, followed by the application of this activity towards biosensing of glucose.

127p runthenight04 02-02-2023 6 3   Download

Laccase-catalyzed polymerization of tyrosine and tyrosine-containing pep-tides was studied in the presence and absence of ferulic acid (FA). Advanced spectroscopic methods such as MALDI-TOF MS, EPR, FTIR microscopy and HPLC-fluorescence, as well as more conventional analyt-ical tools: oxygen consumption measurements and SDS⁄PAGE were used in the reaction mechanism studies. Laccase was found to oxidize tyrosine and tyrosine-containing peptides, with consequent polymerization of the compounds.

11p fptmusic 11-04-2013 35 3   Download

Transcription factors are DNA-binding proteins that regulate key biologi-cal processes. Their interactions with DNA are commonly analyzed with gel-based electrophoretic mobility shift assay (EMSA) using radioactively labeled probes. Within various fields of research, there exists an increasing demand to develop assays with faster sample throughput combined with improved sensitivity, increased analytical range, and precise quantification.

9p vinaphone15 28-02-2013 50 3   Download