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Antibodies using protein
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Tetracycline (TC) is a broad-spectrum antibiotic used in the treatment of several infectious diseases. The excessive use of tetracycline antibiotics, especially tetracyclines in livestock, has raised concerns about residues of tetracycline antibiotics in food that may affect consumers' health.
12p
viamancio
04-06-2024
2
0
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The book is divided into six sections, highlighting selected methods in protein chemistry, nucleic acids, recombinant DNA technology (including forensic-based methods), antibody-based techniques, microscopy and imaging, and the use of animals in biomedical sciences.
142p
vimeyers
29-05-2024
3
2
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Ebook "Synthesis and vaccine evaluation of the tumor associated carbohydrate antigen RM2 from prostate cancer" focuses on the synthesis and vaccine evaluation of the prostate tumor- associated carbohydrate antigen RM2. The author first presents the use of the [1+2+3] one-pot sequential strategy to successfully synthesise the RM2 antigen and its analogues as single stereoisomers in every glycosylation step, producing good yields and stereoselectivity.
116p
tracanhphuonghoa1007
22-04-2024
5
3
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Colorectal cancer (CRC) is the third most prevalent cancer in the world, yet the sensitivity and specificity of biomarkers for CRC diagnosis are insufficient. In the present study, we performed a protein microarray screening method to identify antibody markers for CRC. Inhibitor of growth family 1 (ING1) was identified as a candidate tumor antigen for CRC using protein microarrays (ProtoArray).
14p
visharma
20-10-2023
6
2
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Part 1 book "Rabbit biotechnology - Rabbit genomics, transgenesis, cloning and models" includes content: Improvement of rabbit production, basic methods for experimental rabbits, useful information for rabbit genes, proteins, and antibodies, general physiology of rabbits, rabbit transgenesis, rabbit as a model for the study of human diseases.
72p
oursky05
20-09-2023
2
1
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Part 1 book "ELISA - Methods and protocols" includes content: Flow through assay for detection of antibodies using protein a colloidal gold conjugate as a probe; multiplexed microsphere suspension array based immunoassays; lateral flow immunoassay, immuno pcr assay for sensitive detection of proteins in real time; tyramide signal amplification for immunofluorescent enhancement;.... and other contents.
116p
oursky05
20-09-2023
2
2
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The aim of this work was to develop a competitive enzyme immunoassay technique, to detect the presence of traces of soy in meat products. Specific rabbit polyclonal antiserum against soy protein was used as primary antibody.
6p
viisac
23-09-2023
3
1
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We describe a comprehensive evaluation of 12 Protein A stationary phases for capture of biotherapeutics. We first examine the morphological properties of the stationary phases using a variety of orthogonal techniques including electron microscopy, particle sizing, pressure-flow behavior, and isocratic pulse response.
16p
viginny
23-12-2022
8
3
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The repertoire of complex proteins produced by the host cell during monoclonal antibody (mAb) production has generated a bottleneck in downstream bioprocessing. Low ppm levels of host cell proteins (HCPs) must be achieved atthe downstream purification process stage to generate an end product suitable for use in humans.
11p
viginny
23-12-2022
4
3
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Platform manufacturing processes are widely adopted to simplify and standardize the development and manufacturing of monoclonal antibodies (mAbs). However, there are mAbs that do not conform to a platform design due to instability or other protein properties leading to a negative impact on product quality or process performance (non-platform mAb).
9p
viginny
23-12-2022
7
3
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This work is dealing with the use of polystyrene (PS) nanoparticles as substrates for bioanalytical specific interactions. Different techniques were used for the accurate characterization of the PS nanoparticles of 100 nm and 196 nm before coating them with a layer of antibodies against immunoglobulins of type E (aIgE).
11p
viginny
23-12-2022
12
3
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The adsorption behavior of the model proteins: alpha-Lactalbumin, Bovine Serum Albumin, Lysozyme, and a monoclonal antibody, in single component and in binary mixtures, was investigated on two different hydrophobic interaction chromatography resins using both static and dynamic methods.
12p
viginny
23-12-2022
10
3
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Impact of the column on effluent pH in cation exchange pH gradient chromatography, a practical study
In ionexchange chromatography, the pH gradient mode becomes more and more popular today for the analysis of therapeutic proteins as this mode can provide higher or alternative selectivity to the commonly used salt gradient mode.
7p
viginny
23-12-2022
4
3
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This approach involves the use of multiple isocratic steps, together with the addition of a steep negative gradient segment (with a decrease in mobile phase strength) to "park" a slightly more retained peak somewhere along the column (at a given migration distance), while a slightly less retained compound can be eluted.
9p
viginny
23-12-2022
4
3
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In this paper, we determined the optimal flow rate trajectory during the loading phase of a mAb capture column. For this purpose, a multi-objective function was used, consisting of productivity and resin utilization.
8p
viginny
23-12-2022
6
3
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A computational fluid dynamics method was used for prediction of flow behavior and band profiles of small- and macro-molecule compounds eluting in extra-column volumes (ECV) of an Äkta chromatographic system. The model compounds were: Acetone, bovine serum albumin and an antibody. The construction of ECV was approximated by different types of geometries, starting from the simplest two-dimensional.
10p
viginny
23-12-2022
9
3
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Monoclonal antibodies (mAb) are used as therapeutics and for diagnostics of a variety of diseases, and novel antibodies are continuously being developed to find treatments for new diseases. Therefore, the manufacturing process must accommodate a range of mAb characteristics. Acid-sensitive mAbs can severely compromise product purity and yield in the purification process due to the potential formation of aggregates.
8p
viginny
23-12-2022
12
3
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We evaluated the immunoreactivity profiles of eight commercial anti-host cell protein (anti-HCP) antibodies from different host animals and their antigens used for immunization by an isobaric labeled affinity purification-mass spectrometry (AP-MS) method.
9p
vironald
15-12-2022
11
2
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Biopharmaceuticals are becoming increasingly important in modern healthcare. Monoclonal antibodies (mAb) are one of the most widely used therapeutic proteins and are important for the treatment of cancer and autoimmune diseases, among others.
11p
vironald
15-12-2022
8
2
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Cycle stability is important for preparative chromatography resins. Up to 200 cycles have been reported for Protein A affinity resins when used under optimized operating conditions. Through engineered ligands, alkaline resistant Protein A resins are available that can withstand repeated cleaning-in-place cycles with even 1 M NaOH.
7p
vironald
15-12-2022
5
2
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