Aptamer based biosensors

Codeine is an opium alkaloid with a great potential of abuse among opioid consumers. Detection of codeine is a routine procedure in the military and government of some countries for personnel recruitment. Therefore, a specific, selective, and easy to use method would be an important improvement in such detection procedures. According to previous reports, short single-stranded DNA or RNA sequences with high affinity and specificity to their targets, aptamers, could be used for designing an accurate and specific biosensor for codeine.

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Their use in fields of environmental pollution control and early warning is still in the early stages. Biosensors are classified according to their transduction principle such as optical, electrochemical and piezoelectric or based on their recognition element as immunosensors, apt sensors, genosensors, and enzymatic biosensors, when antibodies, aptamers, nucleic acids, and enzymes are, respectively, used.

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Precise detection of the causal metabolite for disease, pollutant etc causing abnormality in human body and environment is the key to cure the defects caused by them. In this regard biosensors are the novel molecule used to detect a particular molecule with unbeatable precision. These biosensor have a large applications, be it in the field of agriculture, medicine, ecology etc. Detection of pollutant causing severe health hazards is one of the hot topics now a day.

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In this work, a novel aptamer-based electrochemical biosensor was developed for the determination of Ochratoxin A (OTA) by using carbon aerogels (CAs) and methylene blue (MB) as signal amplification strategy.

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This book focuses on the basic electrochemical applications of DNA in various areas, from basic principles to the most recent discoveries. The book comprises theoretical and experimental analysis of various properties of nucleic acids, research methods, and some promising applications.

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