Ascorbate oxidase

A phylogenetic analysis of more than 350 multicopper oxidases (MCOs) from fungi, insects, plants, and bacteria provided the basis for a refined classification of this enzyme family into laccasessensu stricto (basidiomyc-etous and ascomycetous), insect laccases, fungal pigment MCOs, fungal ferroxidases, ascorbate oxidases, plant laccase-like MCOs, and bilirubin oxidases.

19p inspiron33 26-03-2013 54 4   Download

The last step of the biosynthesis ofl-ascorbic acid (vitamin C) in plants and animals is catalyzed by l-gulono-1,4-lactone oxidoreductases, which use bothl-gulono-1,4-lactone and l-galactono-1,4-lactone as substrates. l-Gul-ono-1,4-lactone oxidase is missing in scurvy-prone, vitamin C-deficient ani-mals, such as humans and guinea pigs, which are also highly susceptible to tuberculosis.

11p inspiron33 25-03-2013 54 4   Download

The possible presence of dimeric unfolding intermediates might offer a clue to understanding the relationship between tertiary and quaternary structure formation in dimers. Ascorbate oxidase is a large dimeric enzyme that dis-plays such an intermediate along its unfolding pathway.

11p inspiron33 23-03-2013 33 4   Download

Ascorbate oxidase (AAO) is a large, multidomain, dimeric protein whose folding⁄unfolding pathway is characterized by a complex, multistep pro-cess. Here we used fluorescence correlation spectroscopy to demonstrate the formation of partially folded monomers by pH-induced full dissociation into subunits.

9p cosis54 05-01-2013 46 3   Download