
Báo cáo khoa học: Characterization of monomeric substates of ascorbate oxidase
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Ascorbate oxidase (AAO) is a large, multidomain, dimeric protein whose folding⁄unfolding pathway is characterized by a complex, multistep pro-cess. Here we used fluorescence correlation spectroscopy to demonstrate the formation of partially folded monomers by pH-induced full dissociation into subunits.
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