Cytoplasm

Nghiên cứu nhằm phân tích ảnh hưởng của bất thường bào tương noãn có hạt trung tâm (Centrally located cytoplasmic granular - CLCG) lên tỷ lệ thụ tinh, chất lượng phôi ngày 2 và kết cục thai sinh sống của chu kỳ có hình thái noãn trên.

5p vigrab 28-02-2024 5 2   Download

Bài giảng Vi sinh đại cương: Chương 2 - Đào Hồng Hà cung cấp cho học viên các kiến thức về hình thái, cấu tạo và sinh sản của vi sinh vật; cấu tạo tế bào vi khuẩn; Màng tế bào chất (Cytolasmic membrane); cấu trúc màng tế bào vi khuẩn; tế bào chất (cytoplasm); thể nhân (nuclear body);... Mời các bạn cùng tham khảo chi tiết nội dung bài giảng!

123p bachkhinhdaluu 10-12-2021 33 6   Download

Nicotinamide N-methyltransferase (NNMT), a key cytoplasmic protein in the human body, is accountable to catalyze the nicotinamide (NCA) N1 -methylation through S-adenosyl-L-methionine (SAM) as a methyl donor, which has been linked to many diseases. Although extensive studies have concerned about the biological aspect, the detailed mechanism study of the enzyme function, especially in the part of protein dynamics is lacking.

9p thiencuuchu 27-11-2021 17 2   Download

Kháng thể kháng bào tương của neutrophil (Antineutrophil cytoplasmic autoantibodies (ANCA) là kháng thế trực tiếp lên những hạt của bạch cầu đa nhân trung tính và có liên quan đến phản ứng viêm qua trung gian tham gia của neutrophil. ANCA có liên quan trực tiếp đến các bệnh lý viêm mạch máu.

9p viirene271 20-08-2021 18 2   Download

Viêm mạch máu nhỏ là nhóm bệnh hiếm gặp tại Châu Á, trong đó có viêm mạch máu liên quan đến kháng thể kháng bào tương bạch cầu đa nhân trung tính (anti-neutrophil cytoplasmic antibodies, ANCA) và bệnh kháng thể kháng màng đáy cầu thận.

9p vinayeon2711 09-08-2021 18 2   Download

Hybrid technology in many crops has contributed 20-30 per cent increase in the production and exploitation of heterosis through the development of F1 hybrids which has been recently deployed in rice with yield advantage of 20-25 per cent over best pure lines. The main aim of any plant breeding programme is to develop special high yielding hybrids and success of any plant breeding programme depends on the choice of appropriate genotypes as parents in the hybridization programme.

13p angicungduoc6 22-07-2020 26 2   Download

Protein cytoplasmic tyrosine kinase (BTK) được mã hóa bởi gen BTK. Đột biến trên gen BTK là nguyên nhân gây nên bệnh không gammaglobulin máu liên kết nhiễm sắc thể giới tính X (XLA). Mục tiêu: Phát hiện các đột biến trên gen BTK ở các bệnh nhân mắc bệnh XLA.

4p nutifooddau 18-01-2019 60 2   Download

Chapter 3 (part c) provides knowledge of the cytoplasm. This chapter describe the composition of the cytosol; discuss the structure and function of mitochondria; discuss the structure and function of ribosomes, the endoplasmic reticulum, and the Golgi apparatus, including functional interrelationships among these organelles; compare the functions of lysosomes and peroxisomes.

30p tangtuy07 02-04-2016 49 2   Download

The importance of two putative Zn2+-binding (Asp347, Glu429) and two catalytic (Arg431, Lys354) residues in the tomato leucine aminopeptidase (LAP-A) function was tested. The impact of substitutions at these positions, corresponding to the bovine LAP residues Asp255, Glu334, Arg336, and Lys262, was evaluated in His6–LAP-A fusion proteins expressed in Escherichia coli. Sixty-five percent of the mutant His6–LAP-A proteins were unstable or had complete or partial defects in hexamer assembly or stability. The activity of hexameric His6–LAP-As on Xaa-Leu and Leu-Xaa dipeptides was tested.

11p system191 01-06-2013 39 4   Download

FTIR spectroscopy has been applied to study the coordination structures of Mg2þ and Ca2þ ions bound in Akazara scallop troponin C (TnC), which contains only a single Ca2þ binding site. The region of the COO – antisymmetric stretch provides information about the coordination modes of COO – groups to the metal ions: bidentate, unidentate, or pseudo-bridging. Two bands were observed at 1584 and 1567 cm21 in the apo state, whereas additional bands were observed at 1543 and 1601 cm21 in the Ca2þ-bound and Mg2þ-bound states, respectively.

7p system191 01-06-2013 40 2   Download

Porcine heart cytoplasmic malate dehydrogenase (s-MDH) is a dimeric protein (2 · 35 kDa). We have studied equilibrium unfolding and refolding of s-MDH using activity assay, fluorescence, far-UV and near-UV circular dichroism (CD) spectroscopy, hydrophobic probe-1-anilino8-napthalene sulfonic acid binding, dynamic light scattering, and chromatographic (HPLC) techniques. The unfolding and refolding transitions are reversible and show the presence of two equilibrium intermediate states.

11p system191 01-06-2013 38 4   Download

The human immunodeficiency virus type 1, HIV-1, genome encodes a highly conserved regulatory gene product, Vpr (96 amino acids), which is incorporated into virions in quantities equivalent to those of the viral Gag protein. In infected cells, Vpr is believed to function during the early stages of HIV-1 replication (such as transcription of the proviral genome and migration of preintegration nuclear complex), blocks cells in G2 phase and triggers apoptosis.

10p system191 01-06-2013 45 4   Download

Amplification of N-myc oncogene is a frequent event in advanced stages of human neuroblastoma and correlates with poor prognosis and enhanced neovascularization. Angiogenesis is an indispensable prerequisite for the progression and metastasis of solid malignancies, which is modulated by tumor suppressors and oncogenes. We have addressed the possibility that N-myc oncogene might regulate angiogenesis in neuroblastoma. Here, we report that experimental N-Myc overexpression results in down-regulation of leukemia inhibitory factor (LIF), a modulator of endothelial cell proliferation....

10p system191 01-06-2013 34 4   Download

Bacteroides vulgatus has been shown to be involved in the aggravation of colitis. Previously, we separated two potent virulence factors, capsular polysaccharide (CPS) and lipopolysaccharide (LPS), from a clinical isolate of B. vulgatus and characterized the structure of CPS. In this study, we elucidated the structures of O-antigen polysaccharide (OPS) and lipid A in the LPS. LPS was subjected to weak acid hydrolysis to produce the lipid A fraction and polysaccharide fraction.

7p system191 01-06-2013 50 8   Download

Guanosine triphosphate nucleotide analogues such as GppNHp (also named GMPPNP) or GTPcS are widely used to stabilize rapidly hydrolyzing protein-nucleotide complexes and to investigate biochemical reaction pathways. Here we describe the chemical synthesis of guanosine 5¢-O-(c-amidotriphosphate) (GTPcNH2) and a new synthesis of guanosine 5¢-O-(c-fluorotriphosphate) (GTPcF). The two nucleotides were characterized using NMR spectroscopy and isothermal titration calorimetry. Chemical shift data on 31P, 19F and 1H NMR resonances are tabulated.

9p system191 01-06-2013 32 5   Download

The synthesis of 10 new phosphoenolpyruvate (PEP) analogues with modifications in the phosphate and the carboxylate function is described. Included are two potential irreversible inhibitors of PEP-utilizing enzymes. One incorporates a reactive chloromethylphosphonate function replacing the phosphate group of PEP. The second contains a chloromethyl group substituting for the carboxylate function of PEP. An improved procedure for the preparation of the known (Z)- and (E)-3-chloro-PEP is also given.

11p system191 01-06-2013 34 3   Download

The expression of mitochondrial and hydrogenosomal ADP/ATP carriers (AACs) from plants, rat and the anaerobic chytridiomycete fungus Neocallimastix spec. L2 in Escherichia coli allows a functional integration of the recombinant proteins into the bacterial cytoplasmic membrane. For AAC1 and AAC2 from rat, apparent Km values of about 40 lM for ADP, and 105 lM or 140 lM, respectively, for ATP have been determined, similar to the data reported for isolated rat mitochondria.

10p system191 01-06-2013 36 3   Download

Many groups of proteins play important roles in the cell’s response to various stresses. The molecular chaperone GroEL of Escherichia coli represents one such highly conserved family of stress proteins. We have observed that isolated GroEL complexes from stationary cultures contain various polypeptides that can be released from the chaperonin by GroES and/or ATP, and identified two such polypeptides as the proteins GatY and UP12.

9p system191 01-06-2013 28 3   Download

Quercetin 2,3-dioxygenase (2,3QD) is a copper-containing dioxygenase that catalyses the oxidation of the flavonol quercetin to 2-protocatechuoylphloroglucinol carboxylic acid with concomitant production of carbon monoxide. In contrast to iron dioxygenases, very little is known about copper dioxygenases. We have characterized 2,3QD from the fungus Aspergillus japonicus by electron paramagnetic resonance spectroscopy (EPR). At pH 6.0, 2,3QD shows a mixture of two EPR species. The major form has parameters typical of type 2 Cu sites (g// ¼ 2.330, A// ¼ 13.

9p system191 01-06-2013 31 7   Download

A series of trehalose-based oligosaccharides were isolated from the cytoplasmic fraction of Mycobacterium smegmatis and purified by gel-filtration and paper chromatography and TLC. Their structures were determined by HPLC and GLC to determine sugar composition and ratios, MALDI-TOF MS to measure molecular mass, methylation analysis to determine linkages, 1H-NMR to obtain anomeric configurations of glycosidic linkages, and exoglycosidase digestions followed by TLC to determine sequences and anomeric configurations of the monosaccharides....

8p system191 01-06-2013 36 5   Download