Banana bunchy top disease

Bunchy top disease caused by the banana bunchy top virus (BBTV) is a serious disease in hill banana. Detection of the BBTV infection in the planting material could help in the effective management of the disease. Primers were designed for the PCR amplification of BBTV coat protein (CP). In the present study, to confirm the virus infection and to quantify the viral load in the plants, samples from infected and healthy banana were subjected to PCR and southern blot hybridization.

14p trinhthamhodang1215 23-09-2020 9 1   Download

Molecular detection of Banana Bunchy Top Virus (BBTV) was carried out using nucleic acid based polymerase chain reaction (PCR) method with six different primer pairs for the six components of BBTV (DNA1 to DNA6) genome. Samples were collected from six banana cultivars exhibiting the characteristic symptoms Banana bunchy top disease from eight districts of Assam viz., Jorhat, Golaghat, Sonitpur, Morigaon, Nagaon, Kamrup Metro, Kamrup Rural and Goalpara.

14p gaocaolon5 14-06-2020 9 3   Download