Benchmarking protocol

Single-cell RNA-seq technologies require library preparation prior to sequencing. Here, we present the first report to compare the cheaper BGISEQ-500 platform to the Illumina HiSeq platform for scRNA-seq. We generate a resource of 468 single cells and 1297 matched single cDNA samples, performing SMARTer and Smart-seq2 protocols on two cell lines with RNA spike-ins.

8p vigalileogalilei 27-02-2022 11 1   Download

Chromatin conformation capture (3C)-based technologies have enabled the accurate detection of topological genomic interactions, and the adoption of ChIP techniques to 3C-based protocols makes it possible to identify long-range interactions.

21p viarchimedes 26-01-2022 15 0   Download

Robust protocols and automation now enable large-scale single-cell RNA and ATAC sequencing experiments and their application on biobank and clinical cohorts. However, technical biases introduced during sample acquisition can hinder solid, reproducible results, and a systematic benchmarking is required before entering large-scale data production.

16p viarchimedes 26-01-2022 10 0   Download

A comprehensive AP-vetting model is offered and illustrated using: (i) a preliminary estimator of a reasonable sample size, (ii) two Standard Forecasting Models: The Excel versions of the OLS Linear Two-parameter and the Moving Average Models, and (iii) a Benchmarking protocol.

14p viankara2711 04-12-2019 13 1   Download

Big Data Analytics is a panoply of techniques the principal intention of which is to ferret out dimensions or factors from certain data streamed or available over the WWW. We offer a subset or “second” stage protocol of Big Data Analytics (BDA) that uses these dimensional datasets as benchmarks for profiling related data.

21p kequaidan1 05-11-2019 24 2   Download

The paper considers how to scale up dialogue protocols to multilogue, settings with multiple conversationalists. We extract two benchmarks to evaluate scaled up protocols based on the long distance resolution possibilities of nonsentential utterances in dialogue and multilogue in the British National Corpus. In light of these benchmarks, we then consider three possible transformations to dialogue protocols, formulated within an issue-based approach to dialogue management. We show that one such transformation yields protocols for querying and assertion that fulfill these benchmarks. ...

8p bunbo_1 17-04-2013 46 2   Download

Because this document is a benchmark, it does not cover the important requirements that cannot be benchmarked, such as requirements for integration with existing systems (agent vs. agent-less, transport mechanism, ports and protocols, interface with change control, usability of user interface, storage type, integration with physical security systems, etc.).

9p giamdocamnhac 06-04-2013 44 5   Download