Biochemical identification

Bacterial infections have a substantial impact on global health and can become serious if misdiagnosed with several diseases related to the central nervous, cardiovascular, and respiratory systems. The prognosis in patients with infectious disease strongly depends on early diagnosis and appropriate antibiotic therapy. We aimed to compare the accuracy of genus and species-level identification bacteria using biochemical testing and 16S rRNA sequence analysis.

8p viharuno 03-01-2025 3 2   Download

Campylobacter species are a leading cause of food-borne disease and C. jejuni highlight the most potential public health impact of Campylobacter contamination by pathogens originating from animals or animal products.. The total of 33meat samples comprising 8 from sheep (5) and goat (3) also 25 beef samples were screened by morphological, biochemical and molecular technique.

9p chauchaungayxua11 23-03-2021 22 3   Download

The targeting of proteins to particular subcellular sites is an important principle of the functional organization of cells at themolecular level. In turn, knowledge about the subcellular localizationof aprotein is acharacteristic thatmayprovidea hint as to the function of the protein.

11p tumor12 20-04-2013 36 4   Download

Acetylcholinesterase is an enzyme whose best-known function is to hydro-lyze the neurotransmitter acetylcholine. Acetylcholinesterase is expressed in several noncholinergic tissues. Accordingly, we report for the first time the identification of acetylcholinesterase in human umbilical cord vein endo-thelial cells. Here we further performed an electrophoretic and biochemical characterization of this enzyme, using protein extracts obtained by solubili-zation of human endothelial cell membranes with Triton X-100. ...

11p fptmusic 12-04-2013 48 3   Download

The xylanase inhibitor protein I (XIP-I), recently identified in wheat, inhib-its xylanases belonging to glycoside hydrolase families 10 (GH10) and 11 (GH11). Sequence and structural similarities indicate that XIP-I is related to chitinases of family GH18, despite its lack of enzymatic activity. Here we report the identification and biochemical characterization of a XIP-type inhibitor from rice.

11p awards 06-04-2013 30 3   Download

Vascular endothelial growth factor (VEGF) is a key regu-lator of angiogenesis and post-transcriptional regulation plays a major role in VEGF expression. Both the 5¢-and 3¢-UTR are required for VEGF post-transcriptional regu-lation but factors binding to functional sequences within the 5¢-UTR have not been fully characterized. We report here the identification of complexes, binding to theVEGF mRNA 5¢-and3¢-UTR, that contain cold shock domain (CSD) and polypyrimidine tract binding (PTB) RNA binding proteins. ...

13p dell39 03-04-2013 42 5   Download

Although several proteins undergo tyrosine phosphorylation at the nuclear envelope, we achieved, for the first time, the identification of tyrosine-phos-phorylation sites of a nuclear-membrane protein, emerin, by applying two mass spectrometry-based techniques.

12p inspiron33 25-03-2013 39 4   Download

One of several potential approaches to study mechanisms of action of bio-logically active compounds is to develop their agonists and antagonists. In the present study, we report the identification of the first known molecule antagonizing the activity of the plant hormone cytokinin at the receptor level.

10p vinaphone15 27-02-2013 36 2   Download

In living organisms, genes encoding proteins that contain flavins as a pros-thetic group constitute approximately 2–3% of the total. The fluorescence of flavin cofactors in these proteins is a property that is widely employed for biochemical characterisation. Here, we present a modified Thermofluor  approach called ThermoFAD (Thermofluor  -adapted flavin ad hoc detec-tion system), which simplifies identification of optimal purification and storage conditions as well as high-affinity ligands.

8p viettel02 22-02-2013 39 5   Download

The dominant perciform suborder Notothenioidei is an excellent study group for assessing the evolution and functional importance of biochemical adaptations to temperature. The availability of notothenioid taxa in a wide range of latitudes (Antarctic and non-Antarctic) provides a tool to enable identification of physiological and biochemical characteristics gained and lost during evolutionary history.

12p viettel02 22-02-2013 37 3   Download

In the present study, we report the identification of a new gene from the Bacillus subtilisB3 strain (aatB3), which comprises 1308 bp encoding a 436 amino acid protein with a monomer molecular weight of 49.1 kDa. Phylo-genetic analyses suggested that this enzyme is a member of the Ib subgroup of aspartate aminotransferases

13p cosis54 05-01-2013 55 4   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học General Psychiatry cung cấp cho các bạn kiến thức về ngành y đề tài: Identification of progressors in osteoarthritis by combining biochemical and MRI-based markers...

11p thulanh15 18-10-2011 42 4   Download