Biolistic transformation
-
The relatively low efficiency of biolistic transformation and subsequent integration of multiple copies of the introduced gene/s significantly complicate the genetic modification of wheat (Triticum aestivum) and other plant species.
8p vichoji2711 29-04-2020 9 1 Download
-
Targeted mutagenesis using the Agrobacterium tumefaciens-mediated CRISPR-Cas9 system in common wheat
Recently, the CRISPR/Cas9 system has been widely used to precisely edit plant genomes. Due to the difficulty in Agrobacterium-mediated genetic transformation of wheat, the reported applications in CRISPR/Cas9 system were all based on the biolistic transformation.
12p vishikamaru2711 29-04-2020 6 0 Download
-
Unnecessary weed growth in sugarcane fields forces plants to compete for nutrients and sunlight for survival, which most often leads to significant yield losses. As chemical herbicides cannot differentiate between crop plants and weeds, the development of herbicide-tolerant crops is anticipated.
11p caplock2711 22-02-2019 17 1 Download
-
Yellow stem borer has been identified as a major insect pest of deepwater rice, causing severe yield losses. Bt gene(s) from Bacillus thuringiensis have been proven very effective in pest resistance program. The use of transgenic plants expressing Bt gene(s) is now occupied effective approach to control insect infestation. We have successfully introduced a synthetic cryIA(b) gene into embryogenic calli of a deepwater indica rice variety, Vaidehi, by using the biolistic method of transformation.
6p zingzing09 24-04-2013 53 1 Download
-
Plants that have features and characteristics of other species, other genera, other families and even other kingdoms - such as bacteria and mammals - have fascinated man since the dawn of history. Such phenomena, recorded in several mythologies, were in the realm of miracles and the work of God. Now they seem to become realities, though not exactly as described in the ancient folklore.
392p lyly_5 23-03-2013 50 10 Download