Biological aspects

Determine the fish composition and biological characteristics of gobies (Gobiidae and Eleotridae) using for fishery management and sustainable development in the Mekong Delta in generally and Ben Tre Province in specifically.

19p change01 05-05-2016 66 4   Download

The human b-globin gene locus is the subject of intense study, and over the past two decades a wealth of information has accumulated on how tissue-specific and stage-specific expression of its genes is achieved. The data are extensive and it would be difficult, if not impossible, to formulate a comprehensive model integrating every aspect of what is currently known. In this review, we introduce the fundamental characteristics of globin locus regulation as well as questions on which much of the current research is predicated.

11p system191 01-06-2013 50 4   Download

A comparative study of thermodynamic and kinetic aspects of Cu(II) and Ni(II) binding at the N-terminal binding site of human and bovine serum albumins (HSA and BSA, respectively) and short peptide analogues was performed using potentiometry and spectroscopic techniques. It was found that while qualitative aspects of interaction (spectra and structures of complexes, order of reactions) could be reproduced, the quantitative parameters (stability and rate constants) could not.

9p research12 01-06-2013 43 4   Download

PMAP-36 is a cathelicidin-derived host defence peptide originally deduced by a transcript from pig bone marrow RNA. The expression of the propep-tide in leukocytes, and the structure, antimicrobial activity, and mechanism of action of the mature peptide were investigated. The proform is stored as a dimeric precursor of 38 kDa formed by a dimerization site at its C-ter-minal cysteine residue; it is likely that the mature peptide is dimeric when released.

9p fptmusic 11-04-2013 50 2   Download

Several transcription factors with the function of setting the biological clock in vertebrates have been described. A detailed understanding of their nucleocytolasmic transport properties may uncover novel aspects of the regulation of the circadian rhythm. This assumption led us to perform a systematic analysis of the nuclear import characteristics of the different murine PER and CRY proteins, usingXenopusoocytes and HeLa cells as experimental systems.

11p fptmusic 11-04-2013 26 2   Download

Pulchellin is a type 2 ribosome-inactivating protein isolated from seeds of the Abrus pulchellus tenuiflorusplant. This study aims to obtain active and homogeneous protein for structural and biological studies that will clarify the functional aspects of this toxin. The DNA fragment encoding pulchellin A-chain was cloned and inserted into pGEX-5X to express the recombinant pulchellin A-chain (rPAC) as a fusion protein in Escherichia coli. The deduced amino acid sequence analyses of the rPAC presented a high sequential identity ( 86%) with the A-chain of abrin-c. ...

0p awards 05-04-2013 40 3   Download

Enzyme catalysis in low water containing organic solvents is finding an increasing number of applications in diverse areas.This review focuses on some aspects which have not been reviewed elsewhere.Different strategies for obtaining higher activity and stability in such media are described.In this context, the damaging role of lyophilization and the means of overcoming such effects are discussed.Ultrasoni-cation and microwave assistance are two emerging approa-ches for enhancing reaction rates in low water media.

9p dell39 03-04-2013 66 4   Download

NA helicases are ubiquitous molecular motor proteins which harness the chemical free energy of ATPhydrolysis to catalyze the unwinding of energetically stable duplex DNA, and thus play important roles in nearly all aspects of nucleic acid metabolism, including replication, repair, recombina-tion, and transcription. They break the hydrogen bonds between the duplex helix and move unidirectionally along the bound strand. All helicases are also translocases and DNA-dependentATPases.Most contain conserved helicase motifs that act as an engine to power DNA unwinding. ...

14p dell39 03-04-2013 39 5   Download

Amyloid is anin vivo fibrillar substance containing a fibril protein and several additional molecules. Presently, 25 proteins have been reported as main fibril components. Why just a few proteins form amyloidin vivo is still insufficiently understood. Many fibril proteins appear as fragments of larger precursors and for some types it is not clear whether fragmentation comes before or after fibrillation.

8p dell39 27-03-2013 40 2   Download

Oxidative stress, lipid peroxidation, hyperglycemia-induced glycations and environmental exposures increase the cellular concentrations of aldehydes. A novel aspect of the molecular actions of aldehydes, e.g. acetaldehyde and acrolein, is their reaction with the cysteine ligands of zinc sites in proteins and concomitant zinc release.

11p inspiron33 25-03-2013 33 5   Download

Much work has focused on the electrophysiological properties of transient receptor potential channels. Recently, a novel aspect of importance emerged: the interplay of transient receptor potential channels with the cytoskeleton. Recent data suggest a direct interaction and functional reper-cussion for both binding partners.

16p inspiron33 23-03-2013 49 5   Download

Crystal structures of recombinantLactococcus lactis6-phosphogluconate dehydrogenase (LlPDH) in complex with substrate, cofactor, product and inhibitors have been determined. LlPDH shares significant sequence iden-tity with the enzymes from sheep liver and the protozoan parasite Trypano-soma bruceifor which structures have been reported.

12p inspiron33 23-03-2013 34 5   Download

Environmental stress deleteriously affects every aspect of an ectotherm’s biological function. Frequent exposure of terrestrial insects to temperature variation has thus led to the evolution of protective biochemical and phy-siological mechanisms. However, the physiological mechanisms underlying the positive impact of fluctuating thermal regimes (FTRs) on the fitness and survival of cold-exposed insects have not been studied.

9p galaxyss3 21-03-2013 46 6   Download

The 2006 Global BioMillennium Conference took place in Tbilisi, Georgia, on 13–17 July 2006. The Conference was focused on key aspects of gene expression processes. Characteristic of state-of-the-art research in the life sciences, the invited lectures spanned approaches in cell biology, gene expression, and protein function.

4p galaxyss3 21-03-2013 52 4   Download

Saccharomyces cerevisiaeis known to be able to adapt to the presence of the commonly used food preservative benzoic acid with a large energy expenditure. Some mechanisms for the adaptation process have been sug-gested, but its quantitative energetic and metabolic aspects have rarely been discussed.

12p vinaphone15 28-02-2013 36 2   Download

Replication protein A (RPA) complex has been shown, using bothin vivo and in vitro approaches, to be required for most aspects of eukaryotic DNA metabolism: replication, repair, telomere maintenance and homolo-gous recombination.

20p vinaphone15 27-02-2013 54 3   Download

This minireview covers the research carried out in recent years into differ-ent aspects of the function of the flavoproteins involved in cyanobacterial photosynthetic electron transfer from photosystem I to NADP + , flavodox-in and ferredoxin–NADP + reductase. Interactions that stabilize protein– flavin complexes and tailor the midpoint potentials in these proteins, as well as many details of the binding and electron transfer to protein and ligand partners, have been revealed.

17p vinaphone15 25-02-2013 37 3   Download

Prokaryotes, yeasts and plants synthesize thiamin (vitamin B1) via complex pathways. Animal cells capture the vitamin through specific high-affinity transporters essential for internal thiamin homeostasis. Inside the cells, thi-amin is phosphorylated to higher phosphate derivatives. Thiamin diphos-phate (ThDP) is the best-known thiamin compound because of its role as an enzymatic cofactor.

9p viettel02 22-02-2013 33 3   Download

The temporal aspects of signaling are critical to the function of signals in communications, feedback regulation and control. The production and transduction of biological signals by enzymes comprises an area of central importance and rapid progress in the biomedical sciences.

12p viettel02 20-02-2013 29 2   Download

The small leucine-rich proteoglycans (SLRPs) are involved in many aspects of mammalian biology, both in health and disease. They are now being rec- ognized as key signaling molecules with an expanding repertoire of molecu- lar interactions affecting not only growth factors, but also various receptors involved in controlling cell growth, morphogenesis and immunity.

12p viettel02 19-02-2013 47 3   Download