Biosynthesis genes

Flavonoids, a diverse group of secondary metabolites, are known for their health-promoting properties, including antioxidant, anti-inflammatory, and anticancer activities. Phyllanthus amarus is a medicinal plant with extensive traditional use and it is a rich source of flavonoids. This study aimed to elucidate the gene expression in flavonoid biosynthesis in P. amarus.

7p vifilm 11-10-2024 1 0   Download

New antimicrobials are urgently needed to combat the threat of multidrug-resistant bacteria worldwide. Our study aimed to access the antimicrobial and cytotoxic profiles of endophytic actinomycetes associated with Cinnamomum cassia Presl taken from a mountainous region in the Northwest of Vietnam.

11p viling 27-09-2024 4 2   Download

Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive Streptomyces coelicolor A3(2) has been indicated as a high potential PHA producer, phaC gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome.

12p thiencuuchu 27-11-2021 18 2   Download

Detection and screening of genes related to biosynthesis of substances with protease inhibitory activity from microorganisms associated with sponge collected from the Mien trung sea of Vietnam by metagenomics method. Then, selective gene, expression of in vitro and determination of activity of the expressed protein.

27p capheviahe26 25-01-2021 16 3   Download

Rapeseed (Brassica napus L.) and soybean (Glycine max L.) seeds are rich in both protein and oil, which are major sources of biofuels and nutrition. Although the difference in seed oil content between soybean (~ 20%) and rapeseed (~ 40%) exists, little is known about its underlying molecular mechanism.

15p vishikamaru2711 29-04-2020 9 0   Download

In flowering plants, lipid biosynthesis and transport within anthers is essential for male reproductive success. TaMs1, a dominant wheat fertility gene located on chromosome 4BS, has been previously fine mapped and identified to encode a glycosylphosphatidylinositol (GPI)-anchored non-specific lipid transfer protein (nsLTP).

13p vishikamaru2711 29-04-2020 16 0   Download

Soybean is a paleopolyploid that has undergone two whole genome duplication events. Gene duplication is a type of genomic change that can lead to novel functions of pre-existing genes. Chalcone synthase (CHS) is the plantspecific type III polyketide synthase that catalyzes the first committed step in (iso)flavonoid biosynthesis in plants.

13p vishikamaru2711 29-04-2020 11 0   Download

Starch defines a semicrystalline polymer made of two different polysaccharide fractions. The A- and B-type crystalline lattices define the distinct structures reported in cereal and tuber starches, respectively. Amylopectin, the major fraction of starch, is thought to be chiefly responsible for this semicrystalline organization while amylose is generally considered as an amorphous polymer with little or no impact on the overall crystalline organization.

11p system191 01-06-2013 45 4   Download

Business Unit Cell Cybernetics, Plant Research International, Wageningen, the Netherlands; 2University of Wu¨rzburg, Chair of Food Chemistry, Germany; 3Laboratory of Plant Physiology, Wageningen University, the Netherlands Citrus limon possesses a high content and large variety of monoterpenoids, especially in the glands of the fruit flavedo. The genes responsible for the production of these monoterpenes have never been isolated.

12p system191 01-06-2013 42 7   Download

The long uORF-burdened 5¢UTRs of many genes encoding regulatory proteins involved in cell growth and differentiation contain internal ribosomal entry site (IRES) elements. In a previous study we showed that utilization of the weak IRES of platelet-derived growth factor (PDGF2) is activated during megakaryocytic differentiation.

10p system191 01-06-2013 34 4   Download

The decoy approach against nuclear factor kB (NF-kB) is a useful tool to alter NF-kB dependent gene expression using synthetic oligonucleotides (ODNs) carrying NF-kB specific cis-elements. Unfortunately, ODNs are not stable and need to be extensively modified to be used in vivo or ex vivo. We have previously evaluated the possible use of peptide nucleic acids (PNAs) as decoy molecules. The backbone of PNAs is composed of N-(2-aminoethyl)glycine units, rendering these molecules resistant to both nucleases and proteases.

10p system191 01-06-2013 42 4   Download

4-Coumarate:CoA ligase (4CL) is involved in the formation of coenzyme A thioesters of hydroxycinnamic acids that are central substrates for subsequent condensation, reduction, and transfer reactions in the biosynthesis of plant phenylpropanoids. Previous studies of 4CL appear to suggest that many isoenzymes are functionally equivalent in supplying substrates to various subsequent branches of phenylpropanoid biosyntheses. In contrast, divergent members of a 4CL gene family were identified in soybean (Glycine max L.).

12p research12 01-06-2013 54 5   Download

Previous biochemical studies have indicated that the membrane-bound thyrotropin-releasing hormone (TRH)-degrading enzyme (TRH-DE) from brain and liver and the serum TRH-DE are derived from the same gene. These studies also suggested that the serum enzyme is of liver origin. The present study was undertaken to verify these hypotheses. In different species, a close relationship between the activities of the serum enzyme and the particulate liver enzyme was noticed.

9p research12 01-06-2013 51 3   Download

The Rv0948c gene fromMycobacterium tuberculosisH37Rv encodes a 90 amino acid protein as the natural gene product with chorismate mutase (CM) activity. The protein, 90-MtCM, exhibits Michaelis–Menten kinetics with akcat of 5.5 ± 0.2 s )1 and a Kmof 1500 ± 100lmat 37C and pH 7.5. The 2.0 A˚ X-ray structure shows that 90-MtCM is an alla-helical homodimer (Protein Data Bank ID: 2QBV) with the topology ofEscheri-chia coliCM (EcCM), and that both protomers contribute to each catalytic site.

12p research12 29-04-2013 38 3   Download

Random PCR mutagenesis was applied to theThermus thermophilus xylA gene encoding xylose isomerase. Three cold-adapted mutants were isolated with the following amino-acid substitutions: E372G, V379A (M-1021), E372G, F163L (M-1024) and E372G (M-1026). The wild-type and mutated xylAgenes were cloned and expressed in Escherichia coliHB101 using the vector pGEMÒ-T Easy, and their physicochemical and catalytic properties were determined.

7p research12 29-04-2013 54 3   Download

Phaeodactylum tricornutum is an unicellular silica-less diatom in which eicosapentaenoic acid accumulates up to 30%of the total fattyacids.Thismarinediatomwasused for cloning genes encoding fatty acid desaturases involved in eicosapentaenoic acid biosynthesis. Using a combination of PCR, mass sequencing and library screening, the coding sequences of two desaturases were identified.

9p research12 23-04-2013 45 4   Download

Plant cell wall proteins called expansins are thought to disrupt hydrogen bonding between cell wall polysaccha-rides without hydrolyzing them. We describe here a novel gene with sequence similarity to plant expansins, isolated from the cellulolytic fungus Trichoderma reesei. The pro-tein named swollenin has an N-terminal fungal type cel-lulose binding domain connected by a linker region to the expansin-like domain.

10p research12 23-04-2013 35 4   Download

Aquaporin adipose (AQPap), which we identi®ed from human adipose tissue, is a glycerol channel in adipocyte [Kishida et al. (2000) J. Biol. Chem.275, 20896±20902]. In the current study, we determined the genomic structure of the human AQPap gene, and identi®ed three AQPap-like genes that resembled (95%)AQPap, with little expression in human tissues.

13p research12 23-04-2013 33 2   Download

Previously, a phase-variable epitope was detected in the virulent wild-type strain RC1 of Legionella pneumophila serogroup 1 subgroup OLDA using a lipopolysaccharide-speci®c monoclonal antibody, mAb 2625 [LuÈneberg, E., ZaÈhringer,U.,Knirel,Y.A., Steinmann,D.,Hartmann,M., Steinmetz, I., Rohde, M., Kohl, J. & Frosch, M. (1998) J. Exp. Med.188, 49±60]. In the present study, an isogenic mutant strain, termed 5215, was constructed by deletion of genes involved in the biosynthesis of the mAb 2625 epitope....

13p research12 23-04-2013 29 3   Download

The serotype a-specific polysaccharide antigen ofActinoba-cillus actinomycetemcomitansis an unusual sugar, 6-deoxy-D-talose.Guanosinediphosphate (GDP)-6-deoxy-D-talose is the activated sugar nucleotide form of 6-deoxy-D-talose, which has been identified as a constituent of only a few microbial polysaccharides. In this paper, we identify two genes encoding GDP-6-deoxy-D-talose synthetic enzymes, GDP-a-D-mannose 4,6-dehydratase and GDP-4-keto-6-deoxy-D-mannose reductase, in the gene cluster required for the biosynthesis of serotype a-specific polysaccharide anti-gen from A.

9p tumor12 22-04-2013 30 1   Download