Biosynthesis

1-Hydroxyphenazine (HP) is a heterocyclic nitrogenous compound which reported various potential bioactivities. The current work aimed to utilize soybean residue by-product (SRBP) as a C/N source for the biosynthesis of HP via fermentation and investigated its potent inhibition against some plant pathogen fungi.

7p viengfa 28-10-2024 3 1   Download

Flavonoids, a diverse group of secondary metabolites, are known for their health-promoting properties, including antioxidant, anti-inflammatory, and anticancer activities. Phyllanthus amarus is a medicinal plant with extensive traditional use and it is a rich source of flavonoids. This study aimed to elucidate the gene expression in flavonoid biosynthesis in P. amarus.

7p vifilm 11-10-2024 1 0   Download

New antimicrobials are urgently needed to combat the threat of multidrug-resistant bacteria worldwide. Our study aimed to access the antimicrobial and cytotoxic profiles of endophytic actinomycetes associated with Cinnamomum cassia Presl taken from a mountainous region in the Northwest of Vietnam.

11p viling 27-09-2024 4 2   Download

Polyhydroxyalkanoate (PHA) is stored as an important carbon and energy source in bacterial cells. For biomedical applications, gram-positive bacteria can be better sources of PHAs, since they lack outer membrane lipopolysaccharide. Although gram-positive Streptomyces coelicolor A3(2) has been indicated as a high potential PHA producer, phaC gene that encodes the key enzyme PHA synthase in the metabolic pathway is not determined in its genome.

12p thiencuuchu 27-11-2021 18 2   Download

Detection and screening of genes related to biosynthesis of substances with protease inhibitory activity from microorganisms associated with sponge collected from the Mien trung sea of Vietnam by metagenomics method. Then, selective gene, expression of in vitro and determination of activity of the expressed protein.

27p capheviahe26 25-01-2021 17 3   Download

The methanol extract of P. palatiferum (Nees) Radlk. leaves exhibited a moderate inhibitory effect on the L-3,4-dihydroxyphenylalanine (L-DOPA) oxidase activity of tyrosinase in the melanin biosynthesis pathway. Further study may lead to new bioactive compounds from ethyl acetate and n-hexane fractions.

11p tamynhan5 10-12-2020 28 2   Download

This fraction showed the highest antioxidant activity by dose-dependent free radical scavenging action (IC50 69.50 ± 1.55 µg/mL). R. tomentosa leaf extracts, especially the n-hexane fraction, also exhibited strong antimicrobial activity against Gram-negative and Gram-positive bacteria and fungi as well as moderate inhibitory effect on L-DOPA (L-3,4-dihydroxyphenylalanine) oxidase activity of tyrosinase in the melanin biosynthesis pathway.

9p tamynhan8 04-11-2020 13 1   Download

The anther cuticle, which is primarily composed of lipid polymers, is crucial for pollen development and plays important roles in sexual reproduction in higher plants. However, the mechanism underlying the biosynthesis of lipid polymers in maize (Zea mays. L.) remains unclear.

14p vishikamaru2711 29-04-2020 14 1   Download

Rapeseed (Brassica napus L.) and soybean (Glycine max L.) seeds are rich in both protein and oil, which are major sources of biofuels and nutrition. Although the difference in seed oil content between soybean (~ 20%) and rapeseed (~ 40%) exists, little is known about its underlying molecular mechanism.

15p vishikamaru2711 29-04-2020 10 0   Download

In flowering plants, lipid biosynthesis and transport within anthers is essential for male reproductive success. TaMs1, a dominant wheat fertility gene located on chromosome 4BS, has been previously fine mapped and identified to encode a glycosylphosphatidylinositol (GPI)-anchored non-specific lipid transfer protein (nsLTP).

13p vishikamaru2711 29-04-2020 17 0   Download

Soybean is a paleopolyploid that has undergone two whole genome duplication events. Gene duplication is a type of genomic change that can lead to novel functions of pre-existing genes. Chalcone synthase (CHS) is the plantspecific type III polyketide synthase that catalyzes the first committed step in (iso)flavonoid biosynthesis in plants.

13p vishikamaru2711 29-04-2020 11 0   Download

Overview1.Biosynthesis of terpenes2.Biosynthesis of phenols3.Metabolism of fatty acids4.Metabolism of glucose (glycolysis)5.Anabolic pathways of fatty acidsand carbohydrates.Biosynthesis of acyclic terpenoids.Condensation of isoprene units. Formation of acyclic terpenes.Formation of monoterpenes(examples)

18p nguyenhopgh 24-05-2013 75 7   Download

Ferritin from the spleen of the Antarctic teleost Trematomus bernacchii is composed of a single subunit that contains both the ferroxidase center residues, typical of mammalian H chains, and the carboxylate residues forming the micelle nucleation site, typical of mammalian L chains. Comparison of the amino-acid sequence with those available from lower vertebrates indicates that T. bernacchii ferritin can be classified as an M-type homopolymer. Interestingly, the T. bernacchii ferritin chain shows 85.7% identity with a coldinducible ferritin chain of the rainbow trout Salmo gairdneri....

7p system191 01-06-2013 32 4   Download

Starch defines a semicrystalline polymer made of two different polysaccharide fractions. The A- and B-type crystalline lattices define the distinct structures reported in cereal and tuber starches, respectively. Amylopectin, the major fraction of starch, is thought to be chiefly responsible for this semicrystalline organization while amylose is generally considered as an amorphous polymer with little or no impact on the overall crystalline organization.

11p system191 01-06-2013 45 4   Download

The goal of this study was to determine the effects of a putative specific cytosolic phospholipase A2 inhibitor, arachidonyl trifluoromethyl ketone (AACOCF3), on arachidonic acid (AA) release and lipid mediator biosynthesis by ionophore-stimulated human neutrophils. Initial studies indicated that AACOCF3 at concentrations 0–10 lM did not affect AA release from neutrophils. In contrast, AACOCF3 potently inhibited leukotriene B4 formation by ionophore-stimulated neutrophils (IC50  2.5 lM).

11p system191 01-06-2013 43 4   Download

To examine the role of the distal His42 residue in the catalytic mechanism of pea cytosolic ascorbate peroxidase, two site-directed variants were prepared in which His42 was replaced with alanine (H42A) or glutamic acid (H42E). Electronic spectra of the ferric derivatives of H42A and H42E (pH 7.0, l ¼ 0.10 M, 25.0 °C) revealed wavelength maxima [kmax (nm): 397, 509, % 540sh, 644 (H42A); 404, 516, % 538sh, 639 (H42E)] consistent with a predominantly fiveco-ordinate high-spin iron. The specific activity of H42E for oxidation of L-ascorbate (8.2 ± 0.

11p system191 01-06-2013 39 4   Download

Business Unit Cell Cybernetics, Plant Research International, Wageningen, the Netherlands; 2University of Wu¨rzburg, Chair of Food Chemistry, Germany; 3Laboratory of Plant Physiology, Wageningen University, the Netherlands Citrus limon possesses a high content and large variety of monoterpenoids, especially in the glands of the fruit flavedo. The genes responsible for the production of these monoterpenes have never been isolated.

12p system191 01-06-2013 42 7   Download

The long uORF-burdened 5¢UTRs of many genes encoding regulatory proteins involved in cell growth and differentiation contain internal ribosomal entry site (IRES) elements. In a previous study we showed that utilization of the weak IRES of platelet-derived growth factor (PDGF2) is activated during megakaryocytic differentiation.

10p system191 01-06-2013 34 4   Download

In the biosynthesis of the antiarrhythmic alkaloid ajmaline, polyneuridine aldehyde esterase (PNAE) catalyses a central reaction by transforming polyneuridine aldehyde into epivellosimine, which is the immediate precursor for the synthesis of the ajmalane skeleton. The PNAE cDNA was previously heterologously expressed in E. coli. Sequence alignments indicated that PNAE has a 43% identity to a hydroxynitrile lyase from Hevea brasiliensis, which is a member of the a/b hydrolase superfamily. The catalytic triad, which is typical for this family, is conserved.

8p system191 01-06-2013 35 4   Download

Single-crystal X-ray structure determinations of the complex between the minor-groove binder distamycin and d(GGCCAATTGG) reveal two 1 : 1 binding modes which differ in the orientation of the drug molecule in the minor groove. The two crystals were grown from different crystallization conditions and found to diffract to 2.38 and ˚ 1.85 A, respectively. The structures were refined to completion using SHELXL-93, resulting in a residual R factor of ˚ 20.30% for the 2.38-A resolution structure (including 46 ˚ water molecules) and 19.74% for the 1.

10p system191 01-06-2013 29 5   Download